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To utilize microbial lipases for hydrolysis of milk fat, optimum reaction conditions and characteristics of enzymatic reactions of lipases originated from Rhizopus delemar, Mucor sp., and Candida cylindracea were investigated. Optimum pH and temperature were pH 5.6 and 45℃ for Rhizopus delemar lipase, pH7.5 and 35℃ for Mucor sp. lipase, and pH7.5 and 35℃ for Candida cylindracea lipase. Optimum lipase concentration and optimum substrate concentration were 600-800 units/㎖ and 20 milk fat, regardless of their origin. Km values were 6.06% milk fat for Rhizopus delemar lipase, 7.69% for Mucor sp. lipase and 7.99% for Candida cylindracea lipase. Rate of lipid hydrolysis was Rhizopus delemar lipase$gt;Mucor sp. lipase$gt;Candida cylindracea lipase. As the reaction time was extended, liberation of short chain fatty acids was increased. After 8 hours reaction, capric acid content significantly increased with Candida clindracea lipase, palmitic acid with Mucor sp. lipase and butyric acid with Rhizopus delemar lipase.
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